Researchers develop new 3D technique: Innovation to treat Diabetic Foot Ulcer

Diabetes, a lifelong condition that causes a person's blood sugar level to become too high, is among the top ten causes of deaths worldwide. Diabetic foot ulcer (DFU), is a serious complication of diabetes, affecting approximately 25% of diabetic patients. When identified, over 50 per cent are already infected and over 70 per cent of cases result in lower limb amputation. Queen's University Belfast has come out with a new bandage treatment, known as a scaffold, to treat diabetic foot ulcers & also cost-effective while improving patient outcomes.

The 3D printing method was first demonstrated in the 1980s, and since then, the field of 3D printing (3DP) and bioprinting has grown rapidly in recent years due to technological developments and a greater understanding of the capabilities using these technologies, particularly in the development of scaffolds for tissue engineering applications. In the fabrication of 3D (bio) printed scaffolds, the user is firstly required to design a scaffold using computer-aided design (CAD).

Specialist software can then be used to slice the design prior to printing, providing a set of printing instructions for the printer; each 3DP manufacturer provides the software. A wide range of advantages is offered by 3DP, including the capability to rapidly produce elaborate (scaffold) designs of various sizes at low cost. Moreover, 3D-bioprinted scaffolds have shown the ability to achieve high cell loading and maintain high levels of cell viability while also providing structural support to the wound. 

The treatment strategy required for the effective healing of diabetic foot ulcer (DFU) is a complex process that is requiring several combined therapeutic approaches. As a result, there is a significant clinical and economic burden associated in treating DFU. Furthermore, these treatments are often unsuccessful, commonly resulting in lower-limb amputation. 

Graphical abstract

This new research demonstrates outcomes with significant implications for patient quality of life, as well as decreasing the costs and clinical burden in treating DFU. Recent research has focused on drug-loaded scaffolds to treat DFU. The scaffold structure is a novel carrier for cell and drug delivery that enhances wound healing.

Springler Link Research 

The research, published in Springer Link, was presented by Professor Lamprou at the Controlled Release Society (CRS) Workshop Italy.

Professor Dimitrios Lamprou, a Professor of Biofabrication and Advanced Manufacturing at Queen's School of Pharmacy and corresponding author, explains: "These scaffolds are like windows that enable doctors to monitor the healing constantly. This avoids needing to remove them constantly, which can provoke infection and delay the healing process.

Schematics showing (a) extrusion-based, (b) inkjet-based, and (c) laser-assisted bioprinting technologies

"The 'frame' has an antibiotic that helps to 'kill' the bacteria infection, and the 'glass' that can be prepared by collagen/sodium alginate can contain a growth factor to encourage cell growth. The scaffold has two molecular layers that both play an important role in healing the wound."

What experts say

According to expert Ashok Jhingan , Sr Phycisian & Sr director BLK Max, "the annual incidence of diabetic foot ulcer worldwide is between 9.1 to 26.1 million. Around 15 to 25% of patients with diabetes mellitus will develop a diabetic foot ulcer during their lifetime.

The majority of patients with diabetes who develop foot ulcers are male (more than two-thirds). Furthermore, most of them are between 40 and 70 years of age. Older patients face significantly more complications than younger ones.

Diabetic peripheral neuropathy is a precipitating factor in almost 90% of diabetic foot ulcers. Chronically high glucose (blood sugar) levels damage nerves, including the sensory, motor and autonomic nerves. Diabetic neuropathy also damages the immune system and impairs the body's ability to fight infection.

Infection is a frequent (40%-80%) and costly complication of these ulcers and represents a major cause of morbidity and mortality. It is estimated to be the most common cause of diabetes-related admission to hospital and remains one of the major pathways to lower-limb amputation.

Dr. Jhingan also states that the most common risk factors for ulcer formation include diabetic neuropathy, structural foot deformity and peripheral arterial occlusive disease.New bandage therapy, called scaffold, has been created to treat diabetic foot ulcers (DFT) that is less expensive while also providing better patient outcomes by researchers at Queen's University Belfast.

The scaffolds, which are created by 3D bioprinting, gradually release antibiotics over a period of four weeks to cure the wound. The Journal of Drug Delivery and Translational Research published the study.Diabetes is counted among the top 10 causes of death worldwide. It is a lifelong condition that raises a person's blood sugar levels.

A devastating consequence of diabetes, diabetic foot ulcers (DFU) affect about 25 per cent of diabetic patients. More than 50 per cent of cases when discovered are already infected, and more than 70 per cent of them require lower limb amputation.In order to effectively treat DFU, a complex treatment plan comprising several combined therapeutic approaches is required. As a result, treating DFU entails a severe clinical and financial burden. Amputation of the lower limbs is frequently required since these treatments are often unsuccessful.

This new study shows results that have significantly affected patients' quality of life and reduced the expense and clinical burden of treating DFU. Drug-loaded scaffolds have been the subject of recent research to cure DFU. The scaffold structure is a novel carrier for cells and medications that improves wound healing.

Advantages And Disadvantages Of Commonly Used Bioprinting Technologies

Bioprinting technology	Advantages	Disadvantages
Inkjet-based bioprinting	Low cost High cell loading and viability High resolutions (upto 100 micrometre) Suitable for scale-up activities Allows direct printing of cells and other biologics Suitable for in situ bioprinting applications	Additional processing steps may be required (eg chemical crosslinking) Polymer degradation has been associated with continuous inkjet bioprinting
Extrusion-based bioprinting	Low cost  Higher cell seeding than inkjet-based technologies High cell viabilities Moderate (300-600 micrometre) to high (200 mucrometre) Suitable for production of large scale scaffolds Allows direct printing of cells and other biologics Generally requires low printing temperature and pressures Capable of printing high viscocity materials	High temperatures may be required for high viscocity materials, ruiling out the loading of biologics Additional processing steps may be required (eg chemical crosslinking)
Laser-assisted bioprinting	High-speed printing High resolution (10 micrometre) High cell loading No nozzle required which avoids clogging issues Suitable for in situ bioprinting purposes	Time-consuming preparation of the ribbon for printing More expensive than inkjet and extrusion-based technologies Laser source is a potential disruption to cell viability

 

How to prevent foot ulcers?

Dr. Ashok Jhingan states - wearing clean, dry socks that don't have tight elastic bands, which may restrict blood flow to the foot. Doctors advise people with diabetes to avoid walking barefoot and wearing sandals, which expose your feet to splinters, concrete, or sand, which may scrape or irritate the foot.
Keeping your diabetic wounds clean and free from infection is order number one. Some simple diabetic wound healing products include regular antibiotic ointments like Polysporin or Neosporin.

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Scientists 3D-print living skin with blood vessels

Scientists in the US have created 3D-printed skin complete with blood vessels, an advancement which they hope could be a significant step toward creating grafts that are more like the skin our bodies naturally produce. 

The team of researchers at Rensselaer Polytechnic Institute in New York and Yale School of Medicine combined cells found in human blood vessels with other ingredients, including animal collagen, and printed a skin-like material.

After a few weeks, the cells started to form into vasculature (the arrangement of blood vessels in an organ or part), after which the skin was transplanted onto a mouse. Following this procedure, the team found the skin connected with the animal’s vessels.

“Right now, whatever is available as a clinical product is more like a fancy Band-Aid,” said Pankaj Karande, an associate professor of chemical and biological engineering and member of the Center for Biotechnology and Interdisciplinary Studies (CBIS) at Rensselaer.

“It provides some accelerated wound healing, but eventually it just falls off. It never really integrates with the host cells,” he added.

A significant barrier to the integration between cells has been the absence of a functioning vascular system in the skin grafts. For several years, Karande and his team have been working to tackle this challenge.

In one of the team’s first papers on the subject, the researchers found they could take two types of living human cells, make them into ‘bio-inks’ and print them into a skin-like structure.

Since then, he and his team have been working with researchers from Yale School of Medicine to incorporate vasculature to these cells.

In their latest study, the researchers demonstrated that if they add key elements – including human endothelial cells, which line the inside of blood vessels, and human pericyte cells, which wrap around the endothelial cells – with animal collagen and other structural cells typically found in a skin graft, the cells start communicating and forming a biologically relevant vascular structure within the span of a few weeks.

Karande said: “As engineers working to recreate biology, we’ve always appreciated and been aware of the fact that biology is far more complex than the simple systems we make in the lab.

“We were pleasantly surprised to find that, once we start approaching that complexity, biology takes over and starts getting closer and closer to what exists in nature.”

Once the Yale team grafted it onto a special type of mouse, the vessels from the skin printed by the Rensselaer team began to communicate and connect with the mouse’s own vessels.

“That’s extremely important because we know there is actually a transfer of blood and nutrients to the graft which is keeping the graft alive,” Karande added.

In order to make this process usable at a clinical level, however, the researchers would need to be able to edit the donor cells using CRISPR (gene editing) technology to ensure that the vessels can integrate and be accepted by the patient’s body.

“We are still not at that step, but we are one step closer,” Karande said.

“This significant development highlights the vast potential of 3D bioprinting in precision medicine, where solutions can be tailored to specific situations and eventually to individuals,” said Deepak Vashishth, the director of CBIS. “This is a perfect example of how engineers at Rensselaer are solving challenges related to human health.”

Karande added that more work will need to be done to address the challenges associated with burn patients, which include the loss of nerve and vascular endings.

However, the grafts his team have created bring researchers closer to helping people with more discrete issues such as diabetic or pressure ulcers.

“For those patients, these would be perfect, because ulcers usually appear at distinct locations on the body and can be addressed with smaller pieces of skin,” Karande explained. “Wound healing typically takes longer in diabetic patients and this could also help to accelerate that process.”

this is only for your information, kindly take the advice of your doctor for medicines, exercises and so on.     
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https://gseasyrecipes.blogspot.com. feel free to view for easy, simple and healthy recipes    
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