Novel fungal compound can turn on p53 gene in cancer cells
Cancers cells use a special technique to propagate; they delete their
"programmed death" gene through mutation, "forget" to die when their
lifetime is over, and continue to grow instead. A research team from
Tokyo University of Science has developed a method through which a
fungal compound capable of rearming the self-destruct gene in certain
cancer cells can be artificially produced in marketable quantities,
providing a potential cancer therapeutic strategy.
All human body cells have a certain lifespan, during which they
perform their essential duties. At the end of this lifespan, they reach
senescence and-no longer able to perform those duties-die.
This suicidal death is programmed into their genes through a process called apoptosis, causing them to self-destruct in order to make way for fresh, young, and healthy cells to replace them.
Mutations in a special gene called p53 can sometimes interfere with this process. Caused by aging, ultraviolet light, and various mutagenic compounds, these mutations can disable the apoptosis gene, resulting in "zombie" cells that refuse to die and continue to multiply, spreading the disabled gene and replacing healthy working cells with undying, rapidly growing tumors. This is the disease that we call cancer, and it takes many forms depending on which body cells develop the mutations.
Previously, scientists identified an anticancer compound called FE399 in a species of filamentous fungus called Ascochyta, which is often found afflicting common food crops such as cereals. The compound is a specific group of depsipeptides, a type of amino acid group, and was shown to induce apoptosis in cancerous human cells, particularly colorectal cancer, while they are still in vitro, proving its worth as an anti-cancer chemical.
Unfortunately, due to a variety of chemical complexities, the FE399 compound is not easy to purify, which hindered any plans for its widespread applications in cancer treatment. It was thus clear that extracting FE399 from the fungi naturally would not be a commercially feasible method, and despite the promise of a powerful anticancer drug, research into this particular compound was stalled.
The promise of a new anticancer treatment was tempting, however, and Prof Isamu Shiina, along with Dr Takayuki Tonoi, and his team from the Tokyo University of Science, accepted the challenge.
This suicidal death is programmed into their genes through a process called apoptosis, causing them to self-destruct in order to make way for fresh, young, and healthy cells to replace them.
Mutations in a special gene called p53 can sometimes interfere with this process. Caused by aging, ultraviolet light, and various mutagenic compounds, these mutations can disable the apoptosis gene, resulting in "zombie" cells that refuse to die and continue to multiply, spreading the disabled gene and replacing healthy working cells with undying, rapidly growing tumors. This is the disease that we call cancer, and it takes many forms depending on which body cells develop the mutations.
Previously, scientists identified an anticancer compound called FE399 in a species of filamentous fungus called Ascochyta, which is often found afflicting common food crops such as cereals. The compound is a specific group of depsipeptides, a type of amino acid group, and was shown to induce apoptosis in cancerous human cells, particularly colorectal cancer, while they are still in vitro, proving its worth as an anti-cancer chemical.
Unfortunately, due to a variety of chemical complexities, the FE399 compound is not easy to purify, which hindered any plans for its widespread applications in cancer treatment. It was thus clear that extracting FE399 from the fungi naturally would not be a commercially feasible method, and despite the promise of a powerful anticancer drug, research into this particular compound was stalled.
The promise of a new anticancer treatment was tempting, however, and Prof Isamu Shiina, along with Dr Takayuki Tonoi, and his team from the Tokyo University of Science, accepted the challenge.
We wanted to create a lead compound that could treat colon cancer,
and we aimed to do this through the total synthesis of FE399." Isamu Shiina, Professor, Tokyo University of Science
Total synthesis is the process of the complete chemical synthesis
(production) of a complex molecule using commercially available
precursors, allowing mass production. The results of their extensive
studies will be published in the European Journal of Organic Chemistry.
The team figured that first, the structure of the depsipeptide would
need to be identified. This was simple and could be easily performed
using commercially available and inexpensive materials.
Following this simple start, the subsequent procedures required many
steps and resulted in some small failures when isomers were
unsuccessfully isolated.
However, the team was rewarded for their efforts when, in a major
breakthrough, their mass spectrometry and nuclear magnetic resonance
studies confirmed that a trio of spots on a plate showed identical
chemical signature to the known formula of FE399, meaning that they were
able to successfully recreate FE399 synthetically.
Their technique was found to have an overall yield of 20%, which is quite promising for future large-scale production plans.
We hope that this newly produced compound can provide an
unprecedented treatment option for patients with colorectal cancer, and
thus improve the overall outcomes of the disease and ultimately improve
their quality of life."Isamu Shiina
Further research is needed to test the efficiency of FE399 in the
treatment of other solid and blood-based cancers, and before mass
production, the biological activities and structure of the FE399
molecule will need to be evaluated.
But for now, the team from Tokyo University of Science are thrilled
with their findings, and are positive that their research will help to
improve treatments and therapies for patients with colorectal cancer.
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