New method can quickly identify bacteria that cause infections in hospital patients

The identification of the bacteria can be carried out in almost any hospital analysis laboratory.

In near future, identifying the bacterial species responsible for infections developing in hospital patients will take just a few minutes, thanks to the scientists who developed such an analytical procedure.

Developed by researchers from the Institute of Physical Chemistry of the Polish Academy of Sciences in Warsaw, the main role is played by innovative bio-conjugates — luminescent, magnetic micro-particles coated with appropriately selected bacteriophages.

The detection device used in the new technique for identifying bacteria is a flow cytometer.

“Measurement in the cytometer typically takes about a minute. The result is a graph on which we see how all the bio-conjugates scatter the incident light and emit the fluorescence. Since we know the signal, we should obtain from pure bio-conjugates, and can easily determine whether the sample contains the bacteria we are looking for, and if so, in what concentration,” the researchers noted in a paper published in the journal Bio-conjugate Chemistry.

According to researchers, the identification of the bacteria can be carried out in almost any hospital analysis laboratory and the waiting time for the result is reduced to minutes.

It is important to determine the species of the bacteria ravaging the body of a patient to make treatment successful.

“Faster, better, cheaper — we managed to achieve all of these objectives. This can be seen by any interested party as, in full awareness, we relinquished patent protection,” said Jan Paczesny from the Polish National Science Centre. 

Hospital acquired infections are defined as infections that affect the patient within 2 days of hospital admission or 3 days after discharge. This type of infection is also commonly observed after surgery, which usually occurs within a month of an operation. This is because hospitals are actually hotbeds for a wide range of viruses and bacteria and hence, the possibility of spreading the infection from one person to another is quite high.

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