Researchers find new screening tool for Parkinson’s disease
According to a new study, researchers are using breakthrough
gene-editing technology to develop a new screening tool for Parkinson's
disease, a debilitating degenerative disorder of the nervous system.
The technology allows scientists in the lab to "light up" and then monitor a brain protein called alpha-synuclein that has been associated with Parkinson's.
“Alpha-synuclein is a protein that is normally found in the brain. We all have it but for some reason, when you have Parkinson's the levels become abnormal. So if we can monitor this protein in the cell, we can start to measure what causes it to go up and also what treatments can cause it to go down,” said one of the lead researchers on the project.
The researchers believe their work is a crucial step toward identifying new drug therapies for Parkinson's disease.
He is partnering with doctoral student and scientist to study Parkinson's, which affects motor functions caused by a gradual loss of brain cells.
There are about 60,000 new cases of Parkinson's each year in some countries.
They are using CRISPR Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) gene-editing technology.
The system is one of research's fastest growing biomedical techniques that allows scientists to make specific changes in the DNA of plants and animals while not killing cells.
The system is becoming instrumental in studying genetically based treatments for diseases including cancer and Parkinson's.
Kim, who is also a medical doctor, shared, “It's the most powerful and widely used gene-editing technique in use because it allows us to change the DNA in living cells. The innovation of this method is that it enables us to monitor this gene in real-time without killing the cell. Without the CRISPR Cas-9 method, you would have to extract all the proteins from the cell to measure them, which kills the cell.”
Using the CRISPR technique, the team edited the alpha-synuclein gene and inserted a luminescent tag made from proteins that causes fireflies to light up.
Every time the cell creates the alpha-synuclein protein, the tag gives off a light.
He noted, “More light means an increased level of alpha-synuclein, which would be considered a diseased state.”
The researchers found that measuring light was a reliable method to measure alpha-synuclein production.
“If we take one of these modified cells and treat it with a particular drug, if it doesn't produce light anymore, then this means the drug is a potential treatment for this disease,” he said.
With the engineered cells, the team can screen new and existing drugs to see how they regulate alpha-synuclein level in patients.
“With an easy-to-measure reporter like light production, this will allow us to do high throughput screening, where you can test a large panel of drugs at once,” he said.
With the new technology, the scientists hope to identifying ways to reduce alpha-synuclein production that can possibly prevent Parkinson's or its progression in patients diagnosed with the disease.
The team said research will focus on what aspects of the alpha- synuclein protein kill neurons during Parkinson's disease.
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The technology allows scientists in the lab to "light up" and then monitor a brain protein called alpha-synuclein that has been associated with Parkinson's.
“Alpha-synuclein is a protein that is normally found in the brain. We all have it but for some reason, when you have Parkinson's the levels become abnormal. So if we can monitor this protein in the cell, we can start to measure what causes it to go up and also what treatments can cause it to go down,” said one of the lead researchers on the project.
The researchers believe their work is a crucial step toward identifying new drug therapies for Parkinson's disease.
He is partnering with doctoral student and scientist to study Parkinson's, which affects motor functions caused by a gradual loss of brain cells.
There are about 60,000 new cases of Parkinson's each year in some countries.
The system is one of research's fastest growing biomedical techniques that allows scientists to make specific changes in the DNA of plants and animals while not killing cells.
The system is becoming instrumental in studying genetically based treatments for diseases including cancer and Parkinson's.
Kim, who is also a medical doctor, shared, “It's the most powerful and widely used gene-editing technique in use because it allows us to change the DNA in living cells. The innovation of this method is that it enables us to monitor this gene in real-time without killing the cell. Without the CRISPR Cas-9 method, you would have to extract all the proteins from the cell to measure them, which kills the cell.”
Using the CRISPR technique, the team edited the alpha-synuclein gene and inserted a luminescent tag made from proteins that causes fireflies to light up.
Every time the cell creates the alpha-synuclein protein, the tag gives off a light.
He noted, “More light means an increased level of alpha-synuclein, which would be considered a diseased state.”
The researchers found that measuring light was a reliable method to measure alpha-synuclein production.
“If we take one of these modified cells and treat it with a particular drug, if it doesn't produce light anymore, then this means the drug is a potential treatment for this disease,” he said.
With the engineered cells, the team can screen new and existing drugs to see how they regulate alpha-synuclein level in patients.
“With an easy-to-measure reporter like light production, this will allow us to do high throughput screening, where you can test a large panel of drugs at once,” he said.
With the new technology, the scientists hope to identifying ways to reduce alpha-synuclein production that can possibly prevent Parkinson's or its progression in patients diagnosed with the disease.
The team said research will focus on what aspects of the alpha- synuclein protein kill neurons during Parkinson's disease.
this is only for your information, kindly take the advice of your doctor for medicines, exercises and so on.
https://gscrochetdesigns.blogspot.com. one can see my crochet creations
https://gseasyrecipes.blogspot.com. feel free to view for easy, simple and healthy recipes
https://kneereplacement-stickclub.blogspot.com. for info on knee replacement
Labels: degenerative diseases, nervous system, Parkinson's, protein-alpha-synuclein, screening tool
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