Study unveils how proteins control information processing in brain
A complicated interaction between different proteins is needed for information to pass from one nerve cell to the next. A team of researchers now managed to study this process in the synaptic vesicles, which play an important role in this process.
Several billion nerve cells communicate with each other in the body so that humans and other living beings can perceive and react to their environment. A host of complex chemical and electrical processes occur within a few milliseconds. “ Special messenger substance- known as neurotransmitters- are released at the synapses of the nerve cells. They transmit information between the individual nerve cells,” explains one of the researcher.
The messenger substances are packed into small vesicles called synaptic vesicles, which fuse with the cell membrane in response to an electrical impulse and release the messenger substances. The messenger substances are then recognized by special receptor proteins in the next nerve cell. For this to succeed, numerous proteins have to work together, meshing like cogs in a clockwork mechanism. However, too little is currently known about how this process precisely works, says the researcher.
The researchers have used a special form of mass spectrometry to investigate the process. Cross-linking mass spectrometry helps identify the interaction sites of the proteins. These are mixed with a substance that binds together nearby proteins. This substance reacts at different places depending on how the proteins interact with one another. The mass spectrometer analyses the binding patterns, which can be used to draw conclusions about the arrangement of the proteins. This enables researchers to examine different stages of the vesicles and to detect which protein networks have formed.
The study enables a more thorough understanding of the process of signal transmission in nerve cells. Knowledge about normal process helps scientists recognize and and understand malfunctions that could trigger diseases such as Alzheimer’s.